Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

High-performance purification of histidine-tagged proteins Negligible leakage of Ni²⁺ ions. High binding capacity, at least 40 mg/mL medium Compatible with a wide range of reducing agents, detergents, denaturants and other additives 34 µm bead size for improved resolution

General description

Ni Sepharose^® High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).Ni Sepharose^® High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni²⁺, which will selectively retain proteins with exposed histidine groups.The high stability and broad compatibility of Ni Sepharose^® High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

Legal Information

Sepharose is a registered trademark of Cytiva

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose productsThese products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)